Sfm Particles11/30/2022 ![]() ![]() The SFM image also depended on the orientation of the tip with respect to the specimen. The calibration of the SFM tip with mica provided little clue as to the type of distortion and the amount of lateral broadening observed when the larger gold particles were scanned. Moreover, the distortion of images and broadening of lateral dimensions were specific to the SFM tip used. The measurements of the lateral dimensions of colloidal gold, however, showed substantial differences between SFM and EM in that SFM resulted in an overestimate of the lateral dimensions. The height of the gold particles as measured by SFM and EM was comparable and was little affected by the tip geometry. Images of the colloidal gold particles by SFM were compared with those obtained by electron microscopy (EM). These gold particles deviate little in size, are rigid and have a size similar to biological macromolecules. In this study, spherical colloidal gold particles (10, 20 and 40 nm in diameter) were used to determine (1) tip parameters (size, shape and semi-vertical angle) (2) the distortion of the image caused by the tip and (3) the overestimation or broadening of lateral dimensions. Two major problems that have confronted imaging with the scanning force microscope have been the distortion of the image and overestimation in measurements of lateral size due to the varying geometry and characteristics of the scanning tip. Scanning force microscopy (SFM) holds great promise for biological research. ![]()
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